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Title   자궁경부암 및 임파절 파라핀 포매조직에서의 ras 암유전자의 발현과 중합효소 연쇄반응을 이용한 자궁경부조직에서의 Human papillomavirus 검색 ( Expression of Oncogene Product and Detection of Human Papillomavirus Using Polymerase Chain Reaction in Paraffin - embedded Cervical carcinoma and their Metastatic Ly
Publicationinfo   1993 Jan; 025(01): 15-33.
Key_word   Carcinoma of the uterine cervix, Haman papilloma virus(HPV), ras oncogene, Polymerase chain reaction(PCR)
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Abstract   Carcinoma of the uterine cervix is the most fre#quent neoplasm among women in Korea. A number of factors have been linked to the progression of this disease. Strong epidemiologic evidence suggests that the human papillomaviruses(HPV), especially type 16 and 18, are the most likely candidate agents in the development of cervical cancer. And certain oncogenes have also been implicated in the progression of the disease. Some authours show that HPV DNA cooperates with activated ras in transforming primary cells. Although commonly categorized as be-longing to either the low risk or high risk groups, the prognostic value of HPV types in cervical cancer has been studied only recently, and the results are controversiaL The patients with H- ras gene alteration had been suggested to have poorer prognosis#. Seventy women treated for an invasive squamous cell carcinoma of the uterine cervix and forty-two normal controls during the period from January, 1985 to December, 1985 were studied to investigate the correlation between HPV positivity and ras gene expression in cervical cancer and to determine the prog- nostic value of HPV positivity and ras gene expression, analyzed using HPV amplification by polymerase chain reaction(PCR) and immunohistochemistry, respectively. The prevalence of HPV l6/or 18 in cervical cancer patients was 80.7(27/33)% while for the control group it was 18. 2(6/33)%(p<0.001). The detection rate of HPV 16 DNA was significantly higher in cervical can- cer than in control tissues(75.4% vs 12.1%; p<0.01), and there was no significant difference be- tween the two groups in the detection rate of HPV 18 DNA(17.5% vs 9.1%; p>0.1). Our results did not reveal any statistically significant correlations between HPV positivity and the age, stage, histologic types and lymph node metastases, but statistically significant correlations in tumor size and the invasion depth of cervical walL The ras gene expression was positive in 33.3% of cervical cancer while in only 3.0% of control tissues(p<0.00l). The expression of ras gene was not significantly associated with age, clinical stage, tumor size, the invasion depth of cervical wall and the lymph node metastasis. The association between HPV positivity and ras gene expression was not statistically significant. The 5-year disease free survival(DFS) rates were not significantly different between the cancer patients with positive and negative HPV detection(38.5% vs 34.8%; p>0.1) and between with and without ras gene expression(33.3% vs 42.9%;p >0.1).
저 자   이효표(Hyo Pyo Lee),송용상(Yong Sang Song),김종훈(Jong Hoon Kim),김병기(Byoung Kee Kim),최영민(Young Min Choi),박성희(Seong Hoe Park),강순범(Soon Beom Kang)